Macvector wont let me reverse and complement sequence

(A) Representative image of an mttp c655/c655 mutant embryo expressing eGFP-CAAX in the YSL and a fully opaque yolk. Embryos expressing eGFP-CAAX in the YSL were imaged at 3 dpf, and images were scored for the degree of yolk opacity by a lab member who was blinded to the genotype of the fish. One-cell stage mttp c655/c655 embryos were co-injected with CMV: mttp-FLAG and the CMV: eGFP-CAAX plasmid, or CMV: eGFP-CAAX alone as a control. S2 Fig: Expression of wild-type zebrafish mttp-FLAG rescues the opaque yolk phenotype in mttp c655/c655 embryos. Farber, Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Validation, Writing вЂ“ original draft, Writing вЂ“ review & editing 1, 3, * Mahmood Hussain, Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing вЂ“ original draft, Writing вЂ“ review & editing, 2, * and Steven A. Busch-Nentwich, Funding acquisition, Resources, Supervision, Writing вЂ“ original draft, Writing вЂ“ review & editing, 4, 5 Lloyd Ruddock, Funding acquisition, Resources, Software, Supervision, Visualization, Writing вЂ“ original draft, Writing вЂ“ review & editing, 6 M. Tan, Formal analysis, Visualization, 1 Elisabeth M. Thierer, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Writing вЂ“ original draft, Writing вЂ“ review & editing, 1, 3 Frederick J. Quinlivan, Formal analysis, Investigation, Writing вЂ“ original draft, Writing вЂ“ review & editing, 1 Rosario Recacha, Formal analysis, Visualization, 6 James H.

Hensley, Formal analysis, Investigation, Writing вЂ“ review & editing, 1 Vanessa H. White, Formal analysis, Investigation, Software, Visualization, Writing вЂ“ original draft, Writing вЂ“ review & editing, 4, 5 Monica R. Wilson, Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Validation, Visualization, Writing вЂ“ original draft, Writing вЂ“ review & editing, 1 Sujith Rajan, Formal analysis, Funding acquisition, Investigation, Visualization, Writing вЂ“ original draft, Writing вЂ“ review & editing, 2 Aidan Danoff, Formal analysis, Investigation, 1, 3 Richard J.

Macvector wont let me reverse and complement sequence